I-MsoI (m)

Type

Engineered


Target Sequence

-11  -10  -9   -8   -7   -6   -5   -4   -3   -2   -1   1    2    3    4    5    6    7    8    9    10   11  
C A G A A C G T C G T G A G A C A G T T C C

FASTA Sequence



MTTKNTLQPTEAAYIAGFLDGDGSIYAKLIPRPDYKDIKYQVSLAISFIQRKDKFPYLQDIYDQLGKRGNLRKDR
GDGIADYTIIGSTHLSIILPDLVPYLRIKKKQANRILHIINLYPQAQKNPSKFLDLVKIVDDVQNLNKRADELKS
TNYDRLLEEFLKAGKIESSPTGSGSGSKHPTLTLPTTTSQENLPNGSGSGSGTMTTKNTLQPTEAAYIAGFLDGD
GSIYAKLIPRPDYKDIKYQVSLAISFIQRKDKFPYLQDIYDQLGKRGNLRKDRGDGIADYTIIGSTHLSIILPDL
VPYLRIKKKQANRILHIINLYPQAQKNPSKFLDLVKIVDDVQNLNKRADELKSTNYDRLLEEFLKAGKIESSP

Position Weight Matrix

I-MsoI (m)
posACGT
-11 0.25 0.25 0.25 0.25
-10 1.00 0.81 0.95 0.37
-9 0.51 0.24 1.00 0.29
-8 1.00 0.32 0.68 0.70
-7 1.00 0.72 0.50 0.86
-6 0.44 1.00 0.00 0.95
-5 0.00 0.31 1.00 0.00
-4 0.43 0.50 0.20 1.00
-3 0.24 1.00 0.00 0.33
-2 0.00 0.83 1.00 0.69
-1 1.06 0.97 1.05 1.00
1 0.26 0.02 1.00 0.21
2 1.00 0.81 0.56 0.02
3 0.90 0.00 1.00 0.00
4 1.00 0.00 0.68 0.84
5 0.00 1.00 0.34 0.00
6 1.00 0.00 0.82 0.70
7 0.99 1.14 1.00 1.12
8 0.84 1.15 0.51 1.00
9 0.83 0.94 0.71 1.00
10 0.73 1.00 0.96 1.31
11 0.25 0.25 0.25 0.25

Specificity Changing Mutations (Wild-Type I-MsoI)

Amino Acid Mutations (?)                 Specificity Changes                                          Reference
K28L, T83R -6C to -6G, +6A to +6C 1
I30E, S43R, I85Y -8A to -8G, +8T to +8C 2
K28R, I30E, S43R, N70I, T83R, I85Y -8A to -8G, -7A to -7C, -6C to -6G, +6A to +6C, +7G to +7G, +8T to +8C 2

1     Ashworth J, Havranek JJ, Duarte CM, Sussman D, Monnat RJ Jr, Stoddard BL, Baker D. (2006) Computational redesign of endonuclease DNA binding and cleavage specificity. Nature, 441, 656-659.
2     Ashworth J, Taylor GK, Havranek JJ, Quadri SA, Stoddard BL, Baker D. (2010) Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs. Nucleic Acids Research, 38, 5601-5608.