I-MsoI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
C	A	G	A	A	C	G	T	C	G	T	G	A	G	A	C	A	G	T	T	C	C

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Position Weight Matrix

pos A C G T -11 0.25 0.25 0.25 0.25 -10 1.00 0.81 0.90 0.14 -9 0.59 0.13 1.00 0.15 -8 1.00 0.12 0.67 0.62 -7 1.00 0.61 0.33 0.84 -6 0.54 1.00 0.11 0.92 -5 0.22 0.47 1.00 0.05 -4 0.60 0.65 0.19 1.00 -3 0.21 1.00 0.00 0.35 -2 0.00 0.76 1.00 0.59 -1 0.92 0.99 1.00 0.91 1 0.46 0.18 1.00 0.00 2 1.00 0.76 0.27 0.00 3 0.87 0.00 1.00 0.00 4 1.00 0.00 0.71 0.89 5 0.00 1.00 0.61 0.00 6 1.00 0.15 0.91 0.74 7 0.92 1.00 0.72 0.80 8 0.93 1.00 0.22 0.72 9 0.90 1.00 0.41 0.79 10 0.49 0.91 0.35 1.00 11 0.25 0.25 0.25 0.25

PWM Additional Information

Author: Hui Liu

Notes:
I-MsoI specificity profile generated via ethidium bromide competitive reactions for individual positions.

Specificity Changing Mutations

Amino Acid Mutations (?)	Specificity Changes	Reference
K28L, T83R	-6C to -6G, +6A to +6C	1
I30E, S43R, I85Y	-8A to -8G, +8T to +8C	2
K28R, I30E, S43R, N70I, T83R, I85Y	-8A to -8G, -7A to -7C, -6C to -6G, +6A to +6C, +7G to +7G, +8T to +8C	2

1	Ashworth J, Havranek JJ, Duarte CM, Sussman D, Monnat RJ Jr, Stoddard BL, Baker D. (2006) Computational redesign of endonuclease DNA binding and cleavage specificity. Nature, 441, 656-659.
2	Ashworth J, Taylor GK, Havranek JJ, Quadri SA, Stoddard BL, Baker D. (2010) Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs. Nucleic Acids Research, 38, 5601-5608.

Additional Information

Author: Barry Stoddard

Notes:
1.) Source organism: Monomastix 2.) Host gene: 23S rDNA 3.) Intron-encoded 4.) Cleavage activity verified by many in vitro experiments (Chevalier et al. 2003 J.Mol. Biol. 329: 253-269. 5.) No solubilizing or stabilizing mutations necessary 6.) Structures: 1M5X

Date Added: 2012-01-30