I-SmaMI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
T	A	T	C	C	T	C	C	A	T	T	A	T	C	A	G	G	T	G	T	A	C

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Additional Information

Notes:
1.) Source organism: Sordaria macrospore 2.) Host gene: Cytochrome c oxidase subunit 1, mitochondrial 3.) Intron-encoded 4.) Cleavage activity verified by flow cytometry via the tethered DNA cleavage assay (on the surface of yeast) and by an in vitro cleavage assay (with surface-released protein). Orientation of target site is verified. 5.) Alternative sequence of endonuclease with mutations incorporated that are intended to increase overall solubility for in vitro studies: SKGENSKLNPWAVVGFIDAEGSFMVRVRKNSKYKTGWLVVAIFSVTVDKKDLFLLESLKTFFGGLGSIKKSGNSTFSYRIESSEQLTKIILPFFDKYSLITEKLGDYLLFKKVLELMGTKEHLTQRGLEKIVSLKASINKGLSEELQAAFPQCVPTPRPEINNKNIPDPFWLAGFVSGDGSFKSILKKSESIKVGFQSILVFQITQHARDVKLMESLISYLGCGFIEKDSRGPWLYYTVTNFSDIQGKIIPFFHQYKIIGSKYGDYQDWCKIALIMQNKNHLTPEGLNEIRALKGGMNKGRL 6.) No structure

Date Added: 2011-09-30