I-GzeII

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
A	T	G	G	G	T	A	C	C	A	T	A	T	T	G	G	T	A	C	A	A	A

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Amino Acid Contact Modules

Module	Amino Acid Contact Modules (?)
-11 → -9:	Asn 29, Asp 31*, Lys 32, Arg 34
-8 → -6:	Met 23, His 25, Glu 27, Arg 36, Thr 38, Ser 63, Asn 65, Lys 74, Arg 76
-6 → -4:	Ser 21, Met 23, Gln 40, Lys 42, Asn 65, Ser 67, Val 72, Lys 74
-5 → -3:	Ser 21, Gln 40, Lys 42, Thr 66*, Lys 69, Glu 70, Val 72, Lys 74
+3 → +5:	Ser 179, Ser 181, Arg 196, Ser 198, His 222*, Lys 224*, Ala 228, Ile 230
+4 → +6:	Ser 181, Trp 183, Ser 194, Arg 196, Gly 218, Asn 220, His 222*, Val 232
+6 → +8:	Trp 183, Thr 185, Tyr 190, Ser 192, Ser 194, Gly 218, Arg 234
+9 → +11:	Thr 185, Lys 189*, Tyr 190

Key

	Experiment
	Crystal Structure
	Sequence Alignment
*	Uncertain

Additional Information

Notes:
1.) Source organism: Gibberella zeae 2.) Host gene: NADH:ubiquinone oxidoreductase subunit 2, mitochondrial 3.) Intron-encoded 4.) Cleavage activity verified by flow cytometry via the tethered DNA cleavage assay (on the surface of yeast) and by an in vitro cleavage assay (with surface-released protein). Orientation of target site is verified. 5.) Alternative sequence of endonuclease with mutations incorporated that are intended to increase overall solubility for in vitro studies: DLSTSINPWFVTGFTDAEGSFMIHLEKNKDKWRVRPTFQIKLDIRDKSLLEEIKNYFNNTGSINTSNKECVYKVRSLKDISIIISHFDKYNLITQKKADFELFKKIINKLNSQEHLSYEVGATVLQEIISIRASMNLGLSSSVKEDFPHIIPSNRPLIENMNIPHPEWMAGFVSGEGSFSVYTTSDDKYVSLSFRVSQHNKDKQLLKSFVDFFGCGGFNYHNKGNKAVIFVTRKFEDINDKIIPLFNEYKIKGVKYKDFKDWSKVAKMIESKSHLTTNGYKEICKIKENMNSYRKSSVN 6.) Structure solved (unpublished)

Date Added: 2011-09-30