I-DmoI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
C	G	C	C	G	G	A	A	C	T	T	A	C	C	C	G	G	C	A	A	G	G

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Additional Information

Author: Barry Stoddard

Notes:
1. Host: Desulfurococcus mobilis 2. Host gene: 23S rDNA 3. Intron-encoded 4. Experimentally validated cleavage activity (see PubMed) at hyperthermophilic temperatures 5. Selection to generate mesophilic activity from Dmo yielded: D1--I52F, L95Q D2--I52F, A92T, F101C (Prieto et al. JBC 2008 Feb 15;283(7):4364-74. Epub 2007 Nov 12.) 6. Structures: 1B24, 2VS7, 2VS8

Date Added: 2011-12-31