I-Vdi141I

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
C	T	G	A	C	T	C	T	C	T	T	A	A	G	G	T	A	G	C	C	A	A

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Position Weight Matrix

pos A C G T -11 0.30 1.00 0.40 0.10 -10 0.00 0.00 0.10 1.00 -9 0.00 0.00 1.00 0.00 -8 1.00 0.10 1.00 0.00 -7 0.00 1.00 0.00 0.00 -6 0.10 1.00 1.00 1.00 -5 0.70 1.00 0.20 0.10 -4 0.50 1.00 0.10 1.00 -3 0.00 1.00 0.00 0.00 -2 0.10 0.00 0.00 1.00 -1 0.10 0.10 0.00 1.00 1 1.00 0.00 0.90 0.10 2 1.00 0.00 0.00 0.10 3 0.00 0.00 1.00 0.00 4 0.30 0.05 1.00 0.10 5 0.10 0.20 0.30 1.00 6 1.00 0.30 1.00 0.20 7 0.00 0.00 1.00 0.00 8 0.00 1.00 0.00 0.70 9 0.00 1.00 0.00 0.00 10 1.00 0.10 0.00 0.00 11 1.00 0.30 0.80 0.40

PWM Additional Information

Author: Barry Stoddard

Notes:
PWM generated using side-by-side comparative cleavage assays in vitro as reported in Nomura et al. NAR 2008 35 (22) page 6994 Figure 3.

Additional Information

Author: Barry Stoddard

Notes:
1. Host organism = Vulcanisaeta distributa (hyperthermophilic archae) 2. Host Gene = 23S rDNA 3. Intron encoded 4. Cleavage activity verified via in vitro DNA digests 5. No reported engineered mutations as of 1/1/2012 6. Structure: PDB 3E54

Date Added: 2012-01-01