I-PanMI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
G	C	T	C	C	T	C	A	T	A	A	T	C	C	T	T	A	T	C	A	A	G

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Amino Acid Contact Modules

Module	Amino Acid Contact Modules (?)
-11 → -9:	Asp 30, Lys 32, Tyr 33, Lys 34, Leu 35, Gly 36, Arg 38
-8 → -6:	Met 24, Thr 26, Ile 28, Val 40, Arg 42, Asn 66, Phe 68, Arg 78, Glu 80
-6 → -4:	Ser 22, Met 24, Val 44, Ser 46, Phe 68, Met 70*, Gln 76, Arg 78
-5 → -3:	Ser 22, Val 44, Ser 46, Met 70*, Asp 73*, Ser 74, Gln 76, Arg 78
+3 → +5:	Cys 179, Ser 181, Ile 201, Thr 203, Asp 227, Arg 229*, Tyr 231, Asp 233
+4 → +6:	Ser 181, Val 183, Ser 199, Ile 201, Asn 223, Ser 225, Asp 227, Lys 235
+6 → +8:	Val 183, Phe 185, Ala 195, Lys 197, Ser 199, Asn 223, Thr 237
+9 → +11:	Phe 185, Ser 187, Thr 189*, Ser 190, Lys 191, Leu 192, Gly 193, Ala 195

Key

	Experiment
	Crystal Structure
	Sequence Alignment
*	Uncertain

Additional Information

Notes:
1.) Source organism: Podospora anserina 2.) Host gene: NADH dehydrogenase subunit 3, mitochondrial 3.) Intron-encoded 4.) Cleavage activity verified by flow cytometry via the tethered DNA cleavage assay (on the surface of yeast) and by an in vitro cleavage assay (with surface-released protein). Orientation of target site is verified. 5.) Alternative sequence with shorted N-terminal region for markedly increased activity: STLESKLNPSYISGFVDGEGSFMLTIIKDNKYKLGWRVVCRFVISLHKKDLSLLNKIKEFFDVGNVFLMTKDSAQYRVESLKGLDLIINHFDKYPLITKKQADYKLFKMAHNLIKNKSHLTKEGLLELVAIKAVINNGLNNDLSIAFPGINTILRPDTSLPQILNPFWLSGFVDAEGCFSVVVFKSKTSKLGEAVKLSFILTQSNRDEYLIKSLIEYLGCGNTSLDPRGTIDFKVTNFSSIKDIIVPFFIKYPLKGNKNLDFTDFCEVVRLMENKSHLTKEGLDQIKKIRNRMNTNRK 6.) Structure solved (unpublished)

Date Added: 2011-09-30