I-HjeMI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
T	T	G	A	G	G	A	G	G	T	T	T	C	T	C	T	G	T	T	A	A	T

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Position Weight Matrix

pos A C G T -11 0.25 0.25 0.25 0.25 -10 0.89 0.81 0.83 1.00 -9 0.76 0.51 1.00 0.43 -8 1.00 0.91 0.81 0.97 -7 0.80 0.34 1.00 0.50 -6 0.35 0.56 1.00 0.81 -5 1.00 0.87 1.11 0.96 -4 0.10 0.39 1.00 0.52 -3 0.42 0.02 1.00 0.18 -2 0.34 0.58 0.68 1.00 -1 0.36 0.77 0.04 1.00 1 0.79 0.86 0.69 1.00 2 0.64 1.00 0.21 0.83 3 0.67 0.56 0.74 1.00 4 0.70 1.00 0.91 0.75 5 0.85 0.62 0.70 1.00 6 0.40 0.66 1.00 0.33 7 0.64 0.78 0.52 1.00 8 0.86 0.87 0.79 1.00 9 1.00 0.63 0.68 0.70 10 1.00 0.70 0.85 0.81 11 0.25 0.25 0.25 0.25

Amino Acid Contact Modules

Module	Amino Acid Contact Modules (?)
-11 → -9:	Lys 28*, Tyr 29
-8 → -6:	Ser 22, Thr 24, Lys 26, Thr 31, Glu 33, Lys 57, Ser 59, Arg 72, Arg 74
-6 → -4:	Tyr 20, Ser 22, Gly 35, Glu 37, Ser 59, Arg 61, Ser 70, Arg 72
-5 → -3:	Tyr 20, Gly 35, Glu 37, Arg 61, Glu 67*, Met 68, Ser 70, Arg 72
+3 → +5:	Cys 152, Ser 154, Asp 170, Ala 172, Asp 196*, Cys 200*, Arg 202
+4 → +6:	Ser 154, Tyr 156, Ser 168, Asp 170, Lys 192*, Tyr 194, Asp 196*, Lys 204
+6 → +8:	Tyr 156, Leu 158, Tyr 164*, Ile 166, Ser 168, Lys 192*, Thr 206
+9 → +11:	Leu 158, Tyr 164*

Key

	Experiment
	Crystal Structure
	Sequence Alignment
*	Uncertain

Additional Information

Author: Barry Stoddard

Notes:
1.) Source organism: Hypcrea jecarina 2.) Host gene: mitochondrial cytochrome oxidase (COX) B gene 3.) Intron-encoded 4.) Cleavage activity verified via many in vitro experiments (Jacoby, K. et al. 2012 Nucleic Acids Research) 5.) Additional mutations: see I-AniI 6.) Structure: 3UVF

Date Added: 2012-01-30