I-OnuI (E2)

Type

Engineered

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
G	G	T	C	C	A	C	A	T	A	T	T	T	A	A	C	C	T	T	T	T	G

FASTA Sequence

Simple FASTA Numbered FASTA

Position Weight Matrix

pos A C G T -11 0.25 0.25 0.25 0.25 -10 1.00 0.70 1.00 0.70 -9 0.82 0.83 0.70 1.00 -8 0.13 1.00 -0.03 0.09 -7 0.15 1.00 0.05 0.20 -6 1.00 0.60 0.51 0.46 -5 0.11 1.00 0.23 0.42 -4 1.00 0.38 0.29 0.55 -3 0.02 0.30 0.49 1.00 -2 1.00 0.19 0.02 0.73 -1 0.19 0.61 0.09 1.00 1 0.97 0.56 0.59 1.00 2 0.19 1.22 0.20 1.00 3 1.00 0.65 0.34 0.38 4 1.00 0.90 0.48 0.71 5 0.32 1.00 0.18 0.32 6 0.37 1.00 0.30 0.28 7 0.26 0.28 0.19 1.00 8 0.44 0.26 0.26 1.00 9 0.58 0.25 0.17 1.00 10 0.57 0.40 0.68 1.00 11 0.25 0.25 0.25 0.25

Wild-Type Amino Acid Contact Modules

Module	Amino Acid Contact Modules (?)
-11 → -9:	Asn 32, Lys 34, Ser 35, Ser 36, Val 37, Gly 38, Ser 40
-8 → -6:	Leu 26, Arg 28, Arg 30, Glu 42, Gly 44, Val 68, Ala 70, Lys 80, Thr 82
-6 → -4:	Ser 24, Leu 26, Gln 46, Thr 48, Ala 70, Ser 72, Ser 78, Lys 80
-5 → -3:	Ser 24, Gln 46, Thr 48, Ser 72, Asn 75, Ala 76, Ser 78, Lys 80
+3 → +5:	Cys 180, Phe 182, Ser 201, Thr 203, Lys 227, Lys 229, Trp 234, Asp 236
+4 → +6:	Phe 182, Asn 184, Val 199, Ser 201, Tyr 223, Lys 225, Lys 227, Val 238
+6 → +8:	Asn 184, Ile 186, Gln 195, Gln 197, Val 199, Tyr 223, Thr 240
+9 → +11:	Ile 186, Ser 188, Lys 189, Ser 190, Lys 191, Leu 192, Gly 193, Gln 195

Key

	Experiment
	Crystal Structure
	Sequence Alignment
*	Uncertain