I-OnuI (E2)
Type
Engineered
Target Sequence
-11 | -10 | -9 | -8 | -7 | -6 | -5 | -4 | -3 | -2 | -1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
G | G | T | C | C | A | C | A | T | A | T | T | T | A | A | C | C | T | T | T | T | G |
FASTA Sequence
SAYMSRRESINPWILTGFADAEGSFLLRIRNSNKRSVGYATELGFQICLHIKDKSILENIQSTWKVGVIANSGDN
AVSLRVTRFEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAF
PEIISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSNSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIK
EKNRSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKG
RVF
AVSLRVTRFEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAF
PEIISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSNSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIK
EKNRSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKG
RVF
Position Weight Matrix
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Wild-Type Amino Acid Contact Modules
Module | Amino Acid Contact Modules (?) |
-11 → -9: | Asn 32, Lys 34, Ser 35, Ser 36, Val 37, Gly 38, Ser 40 |
-8 → -6: | Leu 26, Arg 28, Arg 30, Glu 42, Gly 44, Val 68, Ala 70, Lys 80, Thr 82 |
-6 → -4: | Ser 24, Leu 26, Gln 46, Thr 48, Ala 70, Ser 72, Ser 78, Lys 80 |
-5 → -3: | Ser 24, Gln 46, Thr 48, Ser 72, Asn 75, Ala 76, Ser 78, Lys 80 |
+3 → +5: | Cys 180, Phe 182, Ser 201, Thr 203, Lys 227, Lys 229, Trp 234, Asp 236 |
+4 → +6: | Phe 182, Asn 184, Val 199, Ser 201, Tyr 223, Lys 225, Lys 227, Val 238 |
+6 → +8: | Asn 184, Ile 186, Gln 195, Gln 197, Val 199, Tyr 223, Thr 240 |
+9 → +11: | Ile 186, Ser 188, Lys 189, Ser 190, Lys 191, Leu 192, Gly 193, Gln 195 |
Key
Experiment | |
Crystal Structure | |
Sequence Alignment | |
* | Uncertain |