I-OnuI (E2)

Type

Engineered


Target Sequence

-11  -10  -9   -8   -7   -6   -5   -4   -3   -2   -1   1    2    3    4    5    6    7    8    9    10   11  
G G T C C A C A T A T T T A A C C T T T T G

FASTA Sequence



SAYMSRRESINPWILTGFADAEGSFLLRIRNSNKRSVGYATELGFQICLHIKDKSILENIQSTWKVGVIANSGDN
AVSLRVTRFEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAF
PEIISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSNSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIK
EKNRSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKG
RVF

Position Weight Matrix

I-OnuI (E2)


posACGT
-11 0.25 0.25 0.25 0.25
-10 1.00 0.70 1.00 0.70
-9 0.82 0.83 0.70 1.00
-8 0.13 1.00 -0.03 0.09
-7 0.15 1.00 0.05 0.20
-6 1.00 0.60 0.51 0.46
-5 0.11 1.00 0.23 0.42
-4 1.00 0.38 0.29 0.55
-3 0.02 0.30 0.49 1.00
-2 1.00 0.19 0.02 0.73
-1 0.19 0.61 0.09 1.00
1 0.97 0.56 0.59 1.00
2 0.19 1.22 0.20 1.00
3 1.00 0.65 0.34 0.38
4 1.00 0.90 0.48 0.71
5 0.32 1.00 0.18 0.32
6 0.37 1.00 0.30 0.28
7 0.26 0.28 0.19 1.00
8 0.44 0.26 0.26 1.00
9 0.58 0.25 0.17 1.00
10 0.57 0.40 0.68 1.00
11 0.25 0.25 0.25 0.25

Wild-Type Amino Acid Contact Modules

ModuleAmino Acid Contact Modules (?)
-11 → -9:Asn 32, Lys 34, Ser 35, Ser 36, Val 37, Gly 38, Ser 40
-8 → -6:Leu 26, Arg 28, Arg 30, Glu 42, Gly 44, Val 68, Ala 70, Lys 80, Thr 82
-6 → -4:Ser 24, Leu 26, Gln 46, Thr 48, Ala 70, Ser 72, Ser 78, Lys 80
-5 → -3:Ser 24, Gln 46, Thr 48, Ser 72, Asn 75, Ala 76, Ser 78, Lys 80
+3 → +5:Cys 180, Phe 182, Ser 201, Thr 203, Lys 227, Lys 229, Trp 234, Asp 236
+4 → +6:Phe 182, Asn 184, Val 199, Ser 201, Tyr 223, Lys 225, Lys 227, Val 238
+6 → +8:Asn 184, Ile 186, Gln 195, Gln 197, Val 199, Tyr 223, Thr 240
+9 → +11:Ile 186, Ser 188, Lys 189, Ser 190, Lys 191, Leu 192, Gly 193, Gln 195

Key
Experiment
Crystal Structure
Sequence Alignment
* Uncertain