I-SscMI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
A	G	G	T	A	C	C	C	T	T	T	A	A	A	C	C	T	A	T	T	A	A

FASTA Sequence

Simple FASTA Numbered FASTA

Homology Model

PDB File

PyMOL File

Additional Information

Author: Abbie Lambert

Notes:
.) Source organism: Sclerotinia sclerotiorum 2.) Host gene: Small subunit ribosomal rna, mitochondrial 3.) Intron-encoded 4.) Cleavage activity verified by flow cytometry via the tethered DNA cleavage assay (on the surface of yeast) and by an in vitro cleavage assay (with surface-released protein). Orientation of target site is verified. 5.) No solubilizing or stabilizing mutations necessary 6.) No structure

Date Added: 2012-01-30