I-LtrI

Type

Wild-Type

Target Sequence

-11	-10	-9	-8	-7	-6	-5	-4	-3	-2	-1	1	2	3	4	5	6	7	8	9	10	11
A	A	T	G	C	T	C	C	T	A	T	A	C	G	A	C	G	T	T	T	A	G

FASTA Sequence

Simple FASTA Numbered FASTA

Crystal Structure

PDB File

PyMOL File

Position Weight Matrix

pos A C G T -11 0.25 0.25 0.25 0.25 -10 0.24 0.21 0.18 0.36 -9 0.29 0.13 0.19 0.40 -8 0.11 0.85 0.02 0.01 -7 0.04 0.74 0.06 0.16 -6 0.40 0.22 0.24 0.14 -5 0.02 0.75 0.12 0.11 -4 0.38 0.24 0.11 0.28 -3 0.08 0.18 0.45 0.28 -2 0.54 0.01 0.03 0.43 -1 0.18 0.11 0.05 0.66 1 0.22 0.05 0.06 0.67 2 0.06 0.67 0.03 0.24 3 0.44 0.22 0.24 0.10 4 0.29 0.27 0.17 0.26 5 0.20 0.46 0.08 0.26 6 0.22 0.42 0.15 0.20 7 0.16 0.25 0.15 0.44 8 0.26 0.18 0.15 0.41 9 0.30 0.11 0.13 0.47 10 0.18 0.21 0.29 0.32 11 0.25 0.25 0.25 0.25

Amino Acid Contact Modules

Module	Amino Acid Contact Modules (?)
-11 → -9:	Asp 39, Lys 41, Arg 42, Asn 43, Thr 44, Gly 45, Ser 47
-8 → -6:	Met 33, Thr 35, Ser 37, Arg 49, Arg 51, Ile 75, Thr 77, Arg 85, Glu 87
-6 → -4:	Ser 31, Met 33, Arg 53, Gly 55, Thr 77, Arg 83, Arg 85
-5 → -3:	Ser 31, Arg 53, Gly 55, Ile 80*, Asp 81*, Arg 83, Arg 85
+3 → +5:	Ser 186, Tyr 188, Gln 208, Thr 210, Arg 234, Cys 246, Asp 248
+4 → +6:	Tyr 188, Arg 190, Val 206, Gln 208, Asn 230, Arg 232, Arg 234, Val 250
+6 → +8:	Arg 190, Ala 192, Gln 202, Gln 204, Val 206, Asn 230, Thr 252
+9 → +11:	Ala 186, Asn 194*, Thr 196*, Leu 197, Lys 198, Thr 199, Gly 200, Gln 202

Key

	Experiment
	Crystal Structure
	Sequence Alignment
*	Uncertain

Additional Information

Author: Barry Stoddard

Notes:
1.) Source organism: Leptographium truncatum 2.) Host gene: Large subunit ribosomal RNA (rnl), mitochondrial 3.) Intron-encoded fusion protein 4.) Cleavage activity verified by flow cytometry via the tethered DNA cleavage assay (on the surface of yeast) and by an in vitro cleavage assay (with surface-released protein). Orientation of target site is verified. 5.) No solubilizing or stabilizing mutations necessary 6.) Structure: 3R7P

Date Added: 2012-01-30