I-AniI / I-OnuI

Type

Chimera


Target Sequence

-11  -10  -9   -8   -7   -6   -5   -4   -3   -2   -1   1    2    3    4    5    6    7    8    9    10   11  
C T G A G G A G G T T T C A A C C T T T T A

FASTA Sequence



YMSRRESINPWILTGFADAEGYFSLTIKNNNKSSVGYYTTLEFGIELSNKDKSILENIQSTWKVGIISNRGINMV
ALRVRRKEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAFPE
IISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSKSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIKEK
NKSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGRV
F

Position Weight Matrix

I-AniI / I-OnuI


posACGT
-11 0.25 0.25 0.25 0.25
-10 0.16 0.40 0.13 1.00
-9 0.10 0.01 1.00 0.02
-8 1.00 0.19 0.13 0.45
-7 0.02 0.00 1.00 0.01
-6 0.00 0.02 0.65 1.00
-5 1.00 0.80 1.00 1.00
-4 0.00 0.00 1.00 0.08
-3 0.02 0.00 1.00 0.02
-2 0.00 1.00 0.61 0.46
-1 0.23 0.00 0.05 1.00
1 0.31 0.09 0.06 0.53
2 0.00 0.63 0.15 0.22
3 0.37 0.24 0.28 0.11
4 0.27 0.24 0.21 0.27
5 0.27 0.39 0.07 0.27
6 0.24 0.37 0.15 0.24
7 0.13 0.28 0.22 0.37
8 0.30 0.16 0.20 0.34
9 0.23 0.15 0.20 0.42
10 0.22 0.20 0.26 0.31
11 0.25 0.25 0.25 0.25

(-) Endonuclease Amino Acid Contact Modules

ModuleAmino Acid Contact Modules (?)
-11 → -9:Tyr 27
-8 → -6:Ser 20, Thr 22, Gly 25*, Thr 29, Glu 31, Ile 55, Ser 57, Arg 70, Arg 72
-6 → -4:Tyr 18, Ser 20, Gly 33, Glu 35, Ser 57, Arg 59*, Ala 68, Arg 70
-5 → -3:Tyr 18, Gly 33, Glu 35, Arg 59*, Glu 65*, Met 66, Ala 68, Arg 70

(+) Endonuclease Amino Acid Contact Modules

ModuleAmino Acid Contact Modules (?)
+3 → +5:Cys 180, Phe 182, Ser 201, Thr 203, Lys 227, Lys 229, Trp 234, Asp 236
+4 → +6:Phe 182, Asn 184, Val 199, Ser 201, Tyr 223, Lys 225, Lys 227, Val 238
+6 → +8:Asn 184, Ile 186, Gln 195, Gln 197, Val 199, Tyr 223, Thr 240
+9 → +11:Ile 186, Ser 188, Lys 189, Ser 190, Lys 191, Leu 192, Gly 193, Gln 195

Key
Experiment
Crystal Structure
Sequence Alignment
* Uncertain


(-) Endonuclease Specificity Changing Mutations

Amino Acid Mutations (?)                 Specificity Changes                                          Reference
K24N, T29K -8A to -8G 1
Y18W, E35K, R61Q -3G to -3C 1
K24N, G25R, T29I -9G to -9C 1
T29S, E31R, R70L -6G to -6C 1

1     Thyme SB, Jarjour J, Takeuchi R, Havranek JJ, Ashworth J, Scharenberg AM, Stoddard BL, Baker D. (2009) Exploitation of binding energy for catalysis and design. Nature, 461, 1300-1304.