I-AniI / I-OnuI
Type
Chimera
Target Sequence
-11
-10
-9
-8
-7
-6
-5
-4
-3
-2
-1
1
2
3
4
5
6
7
8
9
10
11
C
T
G
A
G
G
A
G
G
T
T
T
C
A
A
C
C
T
T
T
T
A
FASTA Sequence
Simple FASTA
Numbered FASTA
YMSRRESINPWILTGFADAEGYFSLTIKNNNKSSVGYYTTLEFGIELSNKDKSILENIQSTWKVGIISNRGINMV
ALRVRRKEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAFPE
IISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSKSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIKEK
NKSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGRV
F
1 6 11 16 21 26 31 36 41 46 51 56 61 66 71
YMSRRESINPWILTGFADAEGYFSLTIKNNNKSSVGYYTTLEFGIELSNKDKSILENIQSTWKVGIISNRGINMV
76 81 86 91 96 101 106 111 116 121 126 131 136 141 146
ALRVRRKEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAFPE
151 156 161 166 171 176 181 186 191 196 201 206 211 216 221
IISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSKSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCGYIKEK
226 231 236 241 246 251 256 261 266 271 276 281 286 291 296
NKSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGRV
301
F
Position Weight Matrix
pos A C G T
-11
0.25
0.25
0.25
0.25
-10
0.16
0.40
0.13
1.00
-9
0.10
0.01
1.00
0.02
-8
1.00
0.19
0.13
0.45
-7
0.02
0.00
1.00
0.01
-6
0.00
0.02
0.65
1.00
-5
1.00
0.80
1.00
1.00
-4
0.00
0.00
1.00
0.08
-3
0.02
0.00
1.00
0.02
-2
0.00
1.00
0.61
0.46
-1
0.23
0.00
0.05
1.00
1
0.31
0.09
0.06
0.53
2
0.00
0.63
0.15
0.22
3
0.37
0.24
0.28
0.11
4
0.27
0.24
0.21
0.27
5
0.27
0.39
0.07
0.27
6
0.24
0.37
0.15
0.24
7
0.13
0.28
0.22
0.37
8
0.30
0.16
0.20
0.34
9
0.23
0.15
0.20
0.42
10
0.22
0.20
0.26
0.31
11
0.25
0.25
0.25
0.25
(-) Endonuclease Amino Acid Contact Modules
Module Amino Acid Contact Modules (? )
-11 → -9: Tyr 27
-8 → -6: Ser 20 , Thr 22 , Gly 25* , Thr 29 , Glu 31 , Ile 55 , Ser 57 , Arg 70 , Arg 72
-6 → -4: Tyr 18 , Ser 20 , Gly 33 , Glu 35 , Ser 57 , Arg 59* , Ala 68 , Arg 70
-5 → -3: Tyr 18 , Gly 33 , Glu 35 , Arg 59* , Glu 65* , Met 66 , Ala 68 , Arg 70
(+) Endonuclease Amino Acid Contact Modules
Module Amino Acid Contact Modules (? )
+3 → +5: Cys 180 , Phe 182 , Ser 201 , Thr 203 , Lys 227 , Lys 229 , Trp 234 , Asp 236
+4 → +6: Phe 182 , Asn 184 , Val 199 , Ser 201 , Tyr 223 , Lys 225 , Lys 227 , Val 238
+6 → +8: Asn 184 , Ile 186 , Gln 195 , Gln 197 , Val 199 , Tyr 223 , Thr 240
+9 → +11: Ile 186 , Ser 188 , Lys 189 , Ser 190 , Lys 191 , Leu 192 , Gly 193 , Gln 195
Key
Experiment
Crystal Structure
Sequence Alignment
*
Uncertain
(-) Endonuclease Specificity Changing Mutations
Amino Acid Mutations (? )
Specificity Changes
Reference
K24N, T29K
-8A to -8G
1
Y18W, E35K, R61Q
-3G to -3C
1
K24N, G25R, T29I
-9G to -9C
1
T29S, E31R, R70L
-6G to -6C
1